Research Article
Published: 18 February, 2025 | Volume 9 - Issue 1 | Pages: 001-004
Mass serological screening in the Armed Forces involves detecting serological markers of
chronic infections, particularly viral hepatitis B and C, syphilis, and HIV among young military
recruits. The objective of this study is to evaluate the analytical performance of the
chemiluminescence technique (CMIA-Architect i2000 SR) in mass serological screening using the serum-pooling method at the virology laboratory of the Mohammed V Military Teaching Hospital.
Samples with known serological results (positive/negative) were grouped into pools of different sizes (2, 5, 10, and 15 sera). These pools were tested using chemiluminescence (CMIA-Architect i2000 SR). A cost analysis was conducted to assess potential savings based on seroprevalence and pool size.
Results showed that the pooling method maintained 100% specificity. Overall sensitivities for detecting positive samples were 93.1% for HBV, 83.33% for HCV, and 86.36% for HIV. Positive and negative predictive values were high for all three viral markers, highlighting the reliability of the pooling method. Additionally, this approach generated significant cost savings, ranging from 46% to 80%.
Conclusion: This study demonstrated the solid analytical performance of the chemiluminescence technique (CMIA-Architect i 2000 SR) using the serum-pooling method for detecting HBV, HCV, and HIV serological markers in low-seroprevalence regions.
Read Full Article HTML DOI: 10.29328/journal.ijcv.1001062 Cite this Article Read Full Article PDF
Screening; Pooling; Chemiluminescence
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